FP6 priority |
1.1.5 Food Quality and Safety |
Title of the proposal |
Molecular detection of comensals and bacterial pathogens |
Institute |
Slovak Academy of Sciences, Institute of Animal Physiology
Soltesovej 4, 040 01 Kosice, Slovak Republic
www2.saske.sk/iap/ |
Contact |
Name: | Vladimir Kmet, DVM, PhD, DSc, assoc.prof |
Phone: | +421 55 6785075 | E-mail: | kmetv@saske.sk |
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Research subject for a potential FP6 project
Project proposal is oriented on the optimalisation of molecular diagnostics ( PCR, real time PCR, DGGE and FISH) in veterinary and food microbial diagnostics.
The microbial interactions between comensals and pathogens shall be be studied (animal intestinal disorders). As a food matrix shall be used milk and soft cheese, poultry rinsing and meat surface swabs.
As a comensals shall be used aggregative lactobacilli, bifidobacteria, enterococci, anaerobic bacteria (Prevotella, Selenomonas, Fibrobacter) and normal intestinal microflora.
As a pathogens verotoxigenic E.coli (including O157), Listeria monocytogenes , Yersinia enterocolitica, Clostridium perfringens and Campylobacters shall be used. |
Recent international cooperation of the research team
Participation in two 5RP projects: QLK1-CT-1999-00226 „Food PCR“ and QLK2-CT2001-01267 „ genus Clostridium\" |
Proposer´s relevant publications related to the research subject
Lucchini F., Kmet, V., Cesena C., Coppi L., Bottazi V., Morelli L.: Specific detection of a probiotic Lactobacillus strain in faecal samples by using multiplex PCR. FEMS Microb. Lett. 158, 1998, 273-278
Zatkovic B., Molnarova V., Kmet V., Javorsky P., Pristas P.: Diversity of DNA Sequences Among Restriction Endonucleases Producing Selenomonas ruminantium Isolates Detected by Enterobacterial Repetitive Intergenic Consensus Based Polymerase Chain Reaction (ERIC-PCR) . Anaerobe 6, 2000, . 299-304
Holko I., Urbanova J., Kantikova, Pastorova K., Kmet V.: PCR detection of Listeria monocytogenes in milk and milk products - differentiation of suspect isolates. Acta Vet. Brno, 71, 2002, 125-131
Bujnakova D., Kmet V.: Aggregation of Animal Lactobacilli with O157 Enterohemorrhagic Escherichia coli . J. Vet. Med. B., 49, 2002, 152-154 |
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