FP6 priority

1.1.5   Food Quality and Safety

Title of the proposal

Transgenic plants: virus resistance and environmental biosafety

Institute

Slovak Academy of Science, Institute of Virology
Dubravska cesta 9, 842 45 Bratislava, Slovak Republic

Contact

Research subject for a potential FP6 project

The research activities are mainly focused on plant RNA viruses (less viroids, DNA viruses and phytoplasmas).
Experimental studies include the analyses of genome variability and recombina-tion events, pathogenesis, epidemiology, development of diagnostic technologies. Our collection contains several original virus isolates, e.g., AMV, BBSV, PEMV, PPV, natural PPV recombinants , PNRSV and otherwise.
Recently, the plant transformation technologies have been introduced. The experimental systems of tobacco transformed with the alfalfa mosaic virus (AMV) CP gene, PPV P3 gene and gene coding for human alpha 2 interferon, and lucern transformed with AMV CP are under investiga-tion at present.
We have experience with the small-scale field tests too.The research team collaborates with supervising and research institutes of Ministry of Agriculture.

Recent international cooperation of the research team

National Agricultural University, Kiev, Ukraine

Proposer´s relevant publications related to the research subject

Mazurova,L.,Kudela,O.: Transgenic resistance to plant viruses. Proc. of Sem. „ Genetics and breeding of agricultural plants“. Piestany (SR),1998, pp.86-88
Mazurova,L., Subr,Z., Glasa,M., Kudela,O.: Transformation of tobacco plants with the alfalfa mosaic virus T6 coat protein and human alpha 2 IFN genes in sense and antisense orientation. Proc. of Int. Symp.,“Recent Advances in Plant Biotechnology“,Stara Lesna (SR),1999, p. 112Kudela,O., Mazurova,L., Glasa,M., Horvath,L.(1999): Detection of GM plants in food and feed. Testing in Agriculture, 3-4, 36-40.
Horvath,L., Kudela,O. (2001): Present results of seed, feed and food inspection for the presence of GMO. Testing in Agriculture, 1, 43-46.
Glasa,M., Kudela,O., Marie-Jeanne,V., Quiot,J.B. (2001): Evidence of a naturally occurring recombinant isolate of Plum pox virus from Slovakia. Plant Dis. 85, 920