FP6 priority

1.1.1   Genomics and Biotechnology for Health

1.1.1

Title of the proposal

Cloning and characterisation the function of restriction-modification and other nuclease-coding genes from Streptomyces aureofaciens and Streptomyces antibioticus

Institute

Slovak Academy of Sciences, Institute of Molecular Biology
Dubravska cesta 21, 852 01 Bratislava, Slovak Republic

Contact

Research subject for a potential FP6 project

The Project refers to the cloning and analysis of the genes of two NaeI-isoschizomer restriction-modification systems from Streptomyces aureofaciens B96 and S. antibioticus ETH7451 and the study of their homologies, regulation and expression in new host cells. Availability of the gene could allow to investigate the regulation of the expression and to compare this system with that described previously for other methylases, as the EcoRII The other objective will be the analysis of the function of several nuclease and protease genes conceivably related with the hydrolysis of the substrate mycelium DNA and proteins which takes place during the programmed cell death which accompanies the development (differentiation in aerial mycelium) of the former species of Streptomyces. These refer to a nuclease of 18-22 kDa and other of 38-42 kDa which are conceivably related with the processing of substrate mycelium DNA. For this we will purify the proteins and use the information obtained for cloning the corresponding genes. Further on we will investigate their homologies with other nucleases from prokaryotic and eukaryotic organisms and then analyse their function by gene-inactivation experiments. In relation with this objective, we will also consider the possibility of analyse the function of a protease likely related, as postulated for the above nucleases, with the development of the bacterium. The availability of these genes will allow to study further their regulation.

Recent international cooperation of the research team

Collaboration with the group of Dr. Jezus Sanchez,
Area de Microbiologia, Facultad de Medicina, Oviedo, Spain.

Proposer´s relevant publications related to the research subject

1. Godany, A., Pristas, P.,Oktavcova,B.,Farkasovska , J., Ziffova , M., Sevcikova, B. (1996) Characterization of a XhoI izoschizomer in Streptomyces aureofaciens after actinophage infection FEMS Microbiology Letteres, 138, 123 –127
2. Pristas, P., Vanat, I., Godany, A., Javorsky, P.(1994) Restriction endonucleasefrom Selenomonas ruminantum which recognize and cleavage 5-AT/TAAT-3.Arch.Microbiol. 161,5, 449 – 441
3. Godany A., Farkasovska, J., Bukovska, G., Timko J.:2001Connection between Foreign DNA Replication and Induced Expression of the Restriction-Modification System in Streptomyces aureofaciensFolia Microbiol. 46(3), 193-196