The SAS proposals for participation in FP6 projects
are listed in blocks according to the FP6 priority theme structure

FP6 priority
1.1.1   Genomics and Biotechnology for Health
Title of the proposal

Cell swelling induced secretion

Slovak Academy of Sciences, Institute of Experimental Endocrinology
Vlarska 3, 83306 Bratislava, Slovak Republic
Vladimir STRBAK, M.D., DSc.
+421 2 54772709

Research subject for a potential FP6 project

Cell swelling evokes an immediate secretory burst of hormones stored in secretory vesicles with dynamics indistinguishable from those induced by specific secretagogues. In contrast to most types of regulated secretion, that induced by cell swelling in normal cells does not require a rise in intracellular Ca2+ through opening L-type Ca2+ channels.It is highly probable that osmotic effects have a universal influence on exocytosis by all cells. Thus it is possible that a variety of diseases in which the pathophysiology remains obscure, including those with altered neurotransmitter flux, may have abnormalities of exocytosis (or endocytosis) in response to cell volume changes or abnormal transduction mechanisms initiated by cell volume transients as one of their primary etiologic components.Signal transduction leading to cell swelling induced exocytosis remains unknown, our studies indicate a specific pathway which could be elucidated as a part of cell volume regulation project.

Recent international cooperation of the research team

Prof. M.A. Greer, Division of Endocrinology, Department of Medicine, Oregon Health Sciences University, Portland, Oregon

Proposerīs relevant publications related to the research subject

Strbak V., Greer M.: Regulation of Hormone Secretion by Acute Cell Volume Changes: Ca2+-Independent Hormone Secretion. Cellular Physiology and Biochemistry 10: 393-402, 2000.

Benicky J., Nikodemova M., Scsukova S., Zorad S., Strbak V.: Four-week ethanol drinking increases both thyrotropin-releasing hormone (TRH) release and content in rat pancreatic islets. Life Sciences 66: 629-639, 2000.

Kucerova J., Strbak V.: The osmotic component of ethanol and urea action is critical for their immediate stimulation of thyrotropin-releasing hormone (TRH) release from rat brain septum. Physiol Res 50: 309-314, 2001

Nikodemova M., Greer M.A., Strbak V.: Hypo-osmolarity stimulates and high sodium concentration inhibits thyrotropin-releasing hormone secretion from rat hypothalamus. Neuroscience 88: 1299-1306, 1999

Nikodemova M., Greer M.A., Strbak V.: Both iso- and hyperosmotic ethanol stimulate release of hypothalamic thyrotropin-releasing hormone despite opposite effect on neuron volume. Neuroscience 80: 1263-1269, 1997